Question

Describe gene recombination techniques in bacteria. Give diagrams ?

Answer 1

Answer:

Bacterial recombination is a type of genetic recombination in bacteria characterized by DNA transfer from one organism called donor to another organism as recipient. This process occurs in three main ways:

Transformation,

Transduction, and

Conjugation.

Answer 2

$\textbf{Gene Cloning}$ :

1. The carbon copy of an individual is often called a clone. However more appropriately, a clone means to make a genetically exact copy of an organism.

2. In gene cloning, the gene or a piece of DNA fragment is inserted into a bacterial cell where DNA will be multiplied (copied) as the cell divides.

$\textbf{Techniques involved in gene cloning}$ :

$\implies$ Isolation of desired DNA fragment by using restriction enzymes.

$\implies$ Insertion of the DNA fragment into a suitable vector [plasmid] to make rDNA.

$\implies$ Transfer of rDNA into bacterial host cell [Transformation].

$\implies$ Selection and multiplication of recombinant host cell to get a clone.

$\implies$ Expression of cloned gene in host cell.