Describe how you would isolate a mutant that required histidine for growth and was resistant to penicillin.
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My goal is to isolate a mutant that is resistant to the antibiotic penicillin and is a histidine auxotroph. A histidine auxotroph is a cell that is unable to synthesize the amino acid histidine. It contains some mutation in a gene that encodes for a product in the histidine biosynthetic pathway. Thus, a histidine auxotroph cannot grow without the presence of histidine in its growth media.
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