describe in brief about BAC and YAC vectors.
Answers
Answer: YAC
Yeast artificial chromosomes (YACs) are genetically engineered chromosomes derived from the DNA of the yeast, Saccharomyces cerevisiae, which is then ligated into a bacterial plasmid. By inserting large fragments of DNA, from 100–1000 kb, the inserted sequences can be cloned and physically mapped using a process called chromosome walking.
BAC
A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli.[1][2][3] F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division
Explanation:
YACs, BACs, PACs and MACs: artificial chromosomes as research tools. ... Yeast artificial chromosomes (YACs) have become essential research tools as they enable large fragments of DNA to be cloned.
Yeast artificial chromosome (YAC): A vector (carrier) created and used in the laboratory to clone pieces of DNA. A YAC is constructed from the telomeric, centromeric, and replication origin sequences needed for replication in yeast cells.