Describe isolation of DNA, restriction digestion and gel electrophoesis and next one photography
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Isolation of DNA:
The DNA must be recovered from the cells or tissues of the
body (host). Only small amount of tissue
like blood, hair roots, skin, etc. is required.
2. Restriction digestion:
The isolated DNA
is treated with restriction enzymes. Therestrictionenzymes cut the DNA into
small fragments having variable lengths.
This phenomenon is called Restriction
Fragment Length Polymorphism (RFLP).
3. Gel electrophoresis:
The DNA samples are loaded for agarose gel electrophoresis under an electric influence. The DNA flragments, which are negatively charged
move to the positive pole. The movement
of these fragments depends on length of
the fragments. This results in formation of
bands. dsDNA splits into ssDNA by alkali
treatment.
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