Describe the methods for isolation of viruses.
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Answer:
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Viruses can be isolated from samples by filtration. Viral filtrate is a rich source of released virions. ... Animal and plant viruses are detected by cytopathic effects, molecular techniques (PCR, RT-PCR), enzyme immunoassays, and serological assays (hemagglutination assay, hemagglutination inhibition assay).
Explanation:
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Answer:
animal viruses are grown in animals, in embryonated eggs, or in cell culture. (In cell culture, the host animal cells are grown in synthetic medium and then infected with viruses.)
Plant viruses are grown in plants or in plant cell culture. Bacteriophages are grown in susceptible bacteria.
Explanation:
They need living cells for replication, which can be provided by inoculation in live animals among other methods used to culture viruses (cell culture or inoculation of embryonated eggs). Inoculation of human volunteers was the only known method of cultivation of viruses and understanding viral disease.