describe the phenomenon of electrophoresis
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Electrophoresis is a technique used in laboratories in order to separate macromolecules based on size. The technique applies a negative charge so proteins move towards a positive charge. This is used for both DNA and RNA analysis.
Electrophoresis phenomena
Electrophoresis, also called cataphoresis, the movement of electrically charged particles in a fluid under the influence of an electric field. If the liquid rather than the particles is set in motion—e.g., through a fixed diaphragm—the phenomenon is called electroosmosis.
Electrophoresis phenomena
Electrophoresis, also called cataphoresis, the movement of electrically charged particles in a fluid under the influence of an electric field. If the liquid rather than the particles is set in motion—e.g., through a fixed diaphragm—the phenomenon is called electroosmosis.
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(1) Electrophoresis : The movement of colloidal particles under an applied electric potential (or field) is called electrophoresis.
(2) Explanation:Two platinum electrodes are dipped in a colloidal solution filled in U tube. When an electric field (potential) is applied across the electrodes, the charged colloid particles migrate towards oppositely charged electrodes.
(3) Applications :
(a) To decide the electrical charge on the colloidal particles
(b) To separate colloidal particles and obtain a pure true solution.
(c) Since the components of colloidal particles in a mixture migrate with different rates, they can be separated.
(d) The rate of migration of colloidal particles can be measured.
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