describe the technique of paper chromatography
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★ Paper chromatography:-
→ In paper chromatography, a special quality paper known as chromatography paper is used.
→ It acts as the stationary phase. Solution of the mixture is placed at small spot about 2 cm above the end of the chromatographic paper.
→ It is placed in closed jar containing the eluent. As the eluent rises up, the components get separated.
→ When the solvent has moved 3/4th of the chromatographic paper, the paper is removed.
→ If the spots are coloured, they will be visible at different heights from the base line.
→ If the spots are colorless they are observed under UV-light or by using appropriate spray reagent.
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