Question

describe the way of production of virus free plant by shoot tip culture​

Answer 1

Answer:

Shoot tip culture may be described as the culture of terminal (0.1-1.0 mm) portion of a shoot comprising the meristem (0.05-0.1 mm) together with primordial and developing leaves and adjacent stem tissue.Explanation:

(1) Remove the young twigs from a healthy plant. Cut the tip (1 cm) portion of the twig.

(2) Surface sterilize the shoot apices by incuba­tion in a sodium hypochlorite solution (1% available chlorine) for 10 minutes. The ex- plants are thoroughly rinsed 4 times in ster­ile distilled water.

(3) Transfer each explants to a sterilized petri dish.

(4) Remove the outer leaves from each shoot apices with a pair of jeweler’s forceps. This lessens the possibility of cutting into the softer underlying tissues.

(5) After the removal of all outer leaves, the apex is exposed. Cut off the ultimate apex with the help of scalpel and transfer only those less than 1 mm in length to the sur­face of the agar medium or to the surface of filter-paper Bridge. Flame the neck of the culture tube before and after the transfer of the excised tips. Binocular dissecting mi­croscope can be used for cutting the true meristem or shoot tip perfectly.

(6) Incubate the culture under 16hrs light at 25°C.

(7) As soon as the growing single leafy shoot or multiple shoots obtained from single shoot tip or meristem, develop root, transfer them to hormone free medium.

(8) The plantlets formed by this way are later transferred to pots containing compost and kept under greenhouse conditions