Question

design an experiment that you would use to separate cheek cell organelles

Answer 1

Answer:

Human cheek cells

Materials

Glass microscope slides

Plastic cover slips

Paper towels or tissue

Methylene Blue solution (0.5% to 1% (mix approximately 1 part stock solution with 4 parts of water))

Plastic pipette or dropper

Sterile, individually packed cotton swabs

See information on suppliers here.

Methods

Take a clean cotton swab and gently scrape the inside of your mouth.

Smear the cotton swab on the centre of the microscope slide for 2 to 3 seconds.

Add a drop of methylene blue solution and place a coverslip on top. Concentrated methylene blue is toxic if ingested. Wear gloves and do NOT allow children to handle methylene blue solution or have access to the bottle of solution.

Remove any excess solution by allowing a paper towel to touch one side of the coverslip.

Place the slide on the microscope, with 4 x or 10 x objective in position and find a cell. Then view at higher magnification.

Human Cheek CellMethylene blue stains negatively charged molecules in the cell, including DNA and RNA. This dye is toxic when ingested and it causes irritation when in contact with the skin and eyes.

The cells seen are squamous epithelial cells from the outer epithelial layer of the mouth. The small blue dots are bacteria from our teeth and mouth

Answer 2

Human cheek cells

Explanation:

Materials Required

Buccal epithelial cells

Microscope slides

Coverslips

Glycerol

Carbol fuschin stain

Sterile Tooth Picks

Procedure

1. Gently rub inside of the cheek with a sterile toothpick.

2. Transfer the scrapping over in a clean glass slide.

3. Prepare a thin and uniform film of cells on the slide and air dry.

4. Pour the carbol fuschin stain and allow to stand for 2 minutes.

5. wash the slide with distilled water to remove the excess stain.

6. Add a drop of glycerol to prevent it from drying.

7. The cells were observed under microscope.

To learn more:

i)define epithelial tissue. - Brainly.in

https://brainly.in/question/5774460

ii)define epithelial tissue​ - Brainly.in

https://brainly.in/question/15762093