Question

Determination of minimum inhibitory concentrations of antibacterial agents by broth dilution

Answer 1

Abstract Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation, and minimum bactericidal concentrations (MBCs) as the lowest concentration of antimicrobial that will prevent the growth of an organism after subculture on to antibiotic-free media. MICs are used by diagnostic laboratories mainly to confirm resistance, but most often as a research tool to determine the in vitro activity of new antimicrobials,

Answer 2

Explanation:

methods are used to determine the minimum inhibitory concentrations (MICs) of antimicrobial agents and are the reference methods for antimicrobial susceptibility testing against which other methods, such as disk diffusion, are calibrated. MIC methods are widely used in the comparative testing of new agents.