Difference between Fluorescence microscopy and phase contrast microscopy.
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The Phase contrast microscope - The Phase contrast microscope is a light microscope. It is a microscope that works on the technique of enhanced optical contrast which creates contrasting images of the specimens that are transparent. The specimen used will be alive and does not need to be stained. It works on the basic principle of Interference.
Fluorescence microscope - It is also a light microscope that makes use of fluorescence for creating an image. A 3D image is created of the specimen which fluoresces. The specimen is allowed to be coated with a fluorescence material. The specimen absorbs light at a higher wavelength and emits light at a different lower wavelength. It is usually used for studying the images of smaller materials like nucleic acids, to differentiate cells, etc.
Fluorescence microscope - It is also a light microscope that makes use of fluorescence for creating an image. A 3D image is created of the specimen which fluoresces. The specimen is allowed to be coated with a fluorescence material. The specimen absorbs light at a higher wavelength and emits light at a different lower wavelength. It is usually used for studying the images of smaller materials like nucleic acids, to differentiate cells, etc.
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Reliable autofocus is required to obtain accurate measurements of flourescent stained cellular components from a system capable of scanning multiple microscope fields. Autofocus could be performed directly with fluorescent images but due to photobleaching and destructive flouresence by-products. it is best to minimize fluorescence exposure. for photosensitive specimens and live cells. The exposure problem could be completely avoided. by using phase contrast microscopy, implemented through the same optics as fluorescence microscopy.
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