Question

Difference between optical microscope and electron microscope

Answer 1

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$\huge\mathfrak\purple{light\: microscope}$
Illuminating source is the Light.

Specimen preparation takes usually few minutes to hours.

Live or Dead specimen may be seen.

Condenser, Objective and eye piece lenses are made up of glasses.

It has low resolving power (0.25µm to 0.3µm).

It has a magnification of of 500X to 1500X.

The object is 5µm or thicker.

Image is Colored.

Vacuum is not required.

There is no need of high voltage electricity.

There is no cooling system.

$\huge\mathfrak\purple{electron\: microscope}$
Illuminating source is the beam of electrons.

Specimen preparation takes usually takes few days.

Only Dead or Dried specimens are seen.

All lenses are electromagnetic.

It has high resolving power (0.001µm), about 250 times higher  than light microscope.

It has a magnification of 100,000X to 300,000X.

The object is 0.1µm or thinner.

Image is Black and White.

Vacuum is essential for its operation.

High voltage electric current is required (50,000 Volts and above).

It has a cooling system to take out heat generated by high electric current.

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