Question

difference between paper and adsorption chromatography​

Answer 1

Explanation:

Difference between Adsorption and Partition Chromatography

The primary difference is the physical interaction between the sample components and the mobile and stationary phases used.

Adsorption chromatography principle:

Here the sample components physically adsorb (stick) to the stationary phase. There is relatively no adsorption of the sample with the mobile phase. Mobile phase here just forces the sample particles to move over the stationary phase.

adsorption chromatography

If the stationary phase is polar in nature, the polar component of the sample adsorbs to it while others move on.

Adsorption chromatography definition:

It is a process of separation of components of a mixture based on the relative differences in adsorption of components to the stationary phase present in the chromatography column.

Here the molecules or components of the mixture travel with different rates due to differences in their affinity towards stationary phase.

Based on nature, polar compounds adsorb with greater intensity to the polar stationary phase while non-polar compounds remain suspended in the mobile phase. Hence during separation of components, the nonpolar component comes out of the column first while the polar components elute out last due to greater adsorption.

This is exactly reverse on using a non-polar stationary phase.

This adsorption chromatography applies to only solid-liquid or solid-gas chromatography. Because the adsorption phenomenon is an inherent property of solids and hence it is seen only with solid stationary phase chromatography.

Examples for this type are Column chromatography, HPLC chromatography, Thin layer chromatography.

Partition chromatography:

Partition chromatography is a process of separation which is based on the partition coefficient. Here the components of the mixture get distributed into two liquid phases. Here both the stationary phase and mobile phase are liquids. The components get partitioned in between two phase due to the differences in partition coefficients.

Difference between Adsorption and Partition Chromatography

Two immiscible liquids in separate layers due to the partition coefficient

Polar molecules get partitioned into the polar phase to a maximum extent. The nonpolar molecules get partitioned into the non-polar liquid phase.

This mode of partition chromatography applies to Liquid-liquid, liquid-gas chromatography and not to solid-gas chromatography. Gases are freely flow-able hence, for them partition works better than adsorption.

Examples include paper chromatography, gas chromatography, high-performance thin layer chromatography (HPTLC), partition chromatography is the principle of separation.

In paper chromatography, the paper is in the solid state, but the pores in between the paper contain moisture which acts as a stationary liquid phase.

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Answer 2

Answer:

Adsorption chromatography is defined as a type of chromatography in which separation occurs based on adsorption. Partition chromatography is a type of chromatography in which separation is based on partition. Adsorption chromatography is a liquid-solid extraction. Partition chromatography is a liquid-liquid extraction.

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