Difference between preparative hplc and analytical hplc
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Analytical HPLC is usually carried out under high backpressures. For example in Reverse-Phase High Performance Liquid Chromatography by using 250 cm length x 4.6 mm Inner Diameter, and 5 micras C18 columns, and flow rates around 0.5 ml/min of solvents like Acetonitrile (low viscosity) and temperatures about 20 or 30ºC, the minimum backpressure recommended is 11 bar.
Otherwise, preparative and semipreparative chromatography are carried out under low backpressures (1-5 bar) with bigger inner diameter columns and bigger particle size (10 micras in some cases) by passing mobile phases at high flow rates (4-5 ml/min). Alternatively, you can accomplished preparative and semipreparative chromatography by using a short inner diameter and small size particle (5 micras) by passing a very small flow rate (about 0.05 ml/min) mobile phases with very low viscosity (hexane for example) in the aim of keeping a very low backpressure and saving solvents.
In addition, the most important difference between preparative/semipreparative and analytical HPLC is the purpose. Whereas the first one is designed for obtaining the fractionation of groups of compounds (for example fractionation of PAHs and oxy-PAHs) usually as a function of their polarity, analytical HPLC is designed for separation of individual compounds as a function of their medium polarizabilities.
Otherwise, preparative and semipreparative chromatography are carried out under low backpressures (1-5 bar) with bigger inner diameter columns and bigger particle size (10 micras in some cases) by passing mobile phases at high flow rates (4-5 ml/min). Alternatively, you can accomplished preparative and semipreparative chromatography by using a short inner diameter and small size particle (5 micras) by passing a very small flow rate (about 0.05 ml/min) mobile phases with very low viscosity (hexane for example) in the aim of keeping a very low backpressure and saving solvents.
In addition, the most important difference between preparative/semipreparative and analytical HPLC is the purpose. Whereas the first one is designed for obtaining the fractionation of groups of compounds (for example fractionation of PAHs and oxy-PAHs) usually as a function of their polarity, analytical HPLC is designed for separation of individual compounds as a function of their medium polarizabilities.
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