Question

difference between protoplast Culture and tissue Culture,​

Answer 1

Answer:

single cell culture: the growing of individual cell which have been obtained from a tissue callus for suspension culture with the aid of enzymes or mechanically.

protoplast culture: The culture of protoplast obtained from cell by enzymatic digestion of the cell wall.

Answer 2

The differences between protoplast culture and tissue culture are as follows:

(i)

• Tissue culture, also called as, callus culture, is a technique that utilizes the fragments of plants and cultures them in nutrient media under suitable artificial environmental conditions.
• Protoplast culture is the isolation of protoplasts by mechanical and enzymatic methods.

(ii)

• The development of a plant in tissue culture depends on two fundamental principles of cells which include totipotency and plasticity.

• The application of protoplast culture includes the development of hybrids, cell cloning, developing genetically transformed plants and membrane studies.

(iii)

• In tissue culture, segments of different plant parts, known as explants, are cultured. The explants that are used in tissue culture include leaf, stem, root, petiole, hypocotyl, cotyledon, embryo, or meristem.

• The methods used to perform the protoplast culture include hanging-drop cultures, micro-culture chambers and soft agar matrix.