Difference between recombinant cells from non-recombinants on the basis of their ability to produce colour in presence of a chromogenic substance. In this process an enzyme gene become inactivate which is reffered as -
(1) Insertional inactivation
(2) Insertional activation
(3) Chromosomal abberation
(4) Inverson
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Answers
Answer:
those dna with foreign gene is called recombinant dna n those which doesnt contains foreign gene is called nonrecombinant dna Recombinant is a general name given when a piece of one DNA is combined with another DNA giving rise to a new DNA. It is an organism or cell in which genetic recombination has taken place. It is produced by methods of genetic engineering.
When there is no foreign DNA insertion in the original genome, the structure is called non recombinant.
Answer:
Alternative selectable markers have been developed which differentiate recombinants from the non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, ( -galactosidase. This results into inactivation of the enzyme, which is referred i to as insertional inactivation. The presence of a chromogenic j substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. Presence of insert results into insertional inactivation of the -galactosidase and the colonies do not produce any colour, these are identified as recombinant colonies.