Question

Difference between sanger and maxam gilbert and pyrosequencing

Answer 1

Nucleotides are the basic structural units and building blocks of DNA. DNA molecule is composed of a polynucleotide chain. There are four different nucleotides found in DNA. These nucleotides are composed of four different nitrogenous bases named A (adenine), G (guanine), C (cytosine), T (thymine). The order of the nucleotides in the DNA molecule has a great importance as it encodes an important genetic information for organisms growth and development. DNA sequencing refers to the process which determines the precise nucleotide sequence of the DNA. There are different DNA sequencing methods. Maxam Gilbert sequencing and Sanger DNA sequencing are two methods of DNA sequencing which belong to first generation sequencing. Maxam Gilbert sequencing procedure determines the base sequence by chemically cleaving the 5’ end labelled DNA fragments preferentially at each of four nucleotides and gel electrophoresis. Sanger sequencing procedure determines the nucleotide sequence by synthesizing single-stranded DNA using DNA polymerase and dideoxynucleotides and gel electrophoresis.This is the key difference between Maxam Gilbert and Sanger Sequencing.