Difference between t24 cell lines and 5637 cell lines
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Answer:
Selecting the appropriate cell line
Consider the following criteria for selecting the appropriate cell line for your experiments:
Species: Non-human and non-primate cell lines usually have fewer biosafety restrictions, but ultimately your experiments will dictate whether to use species-specific cultures or not.
Functional characteristics: What is the purpose of your experiments? For example, liver- and kidney-derived cell lines may be more suitable for toxicity testing.
Finite or continuous: While choosing from finite cell lines may give you more options to express the correct functions, continuous cell lines are often easier to clone and maintain.
Normal or transformed: Transformed cell lines usually have an increased growth rate and higher plating efficiency, are continuous, and require less serum in media, but they have undergone a permanent change in their phenotype through a genetic transformation.
Growth conditions & characteristics: What are your requirements with respect to growth rate, saturation density, cloning efficiency, and the ability to grow in suspension? For example, to express a recombinant protein in high yields, you might want to choose a cell line with a fast growth rate and an ability to grow in suspension.
Other criteria: If you are using a finite cell line, are there sufficient stocks available? Is the cell line well-characterized, or do you have to perform the validation yourself? If you are using an abnormal cell line, do you have an equivalent normal cell line that you can use as a control? Is the cell line stable? If not, how easy it is to clone it and generate sufficient frozen stocks for your experiments?