different diapause and non diapause egg
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In diapause eggs, embryos ceased to develop at the late gastrula stage (approximately 72 hr after oviposition), but in non-diapause eggs, lar- vae hatched 11 days after oviposition. ... After injection, the eggs were incubated in a wet chamber at 25°C for 3 hr and stored at –80°C until the extraction of metabolites.
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