Question

different strategies for purification of antimicrobial peptides from lactic acid bacteria (lab)

Answer 1

Different strategies for purification of antimicrobial peptides

from Lactic Acid Bacteria (LAB)

E. Vera Pingitore, E. Salvucci, F. Sesma, and M. E. Nader-Macías*

Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145 (4000) San Miguel de

Tucumán, Tucumán, Argentina

Bacteriocins are natural peptides secreted by several bacteria that exert bactericidal activity against other

bacterial species. Research in this topic is promoted not only by the need to develop alternatives to

antibiotics and drugs that have shown non-desirable effects, but also to the capacity of some bacteriocin to

inhibit saprophytic and food-borne pathogens in food stuffs. Many LAB are bacteriogenics, and the

purification of the bacteriocins produced is helpful for the knowledge of the mechanism of action,

structure, and other characteristics, which helps to isolate the bacteriocin biosynthetic genes. In this

chapter, different techniques applied to purify some bacteriocins from LAB are described. The most

frequently applied techniques involve salt precipitation followed by various combinations of ion-exchange

(IEC) and reverse phase C18 solid phase extraction, absorption-desorption (AD), reverse-phase high-

performance liquid chromatography (RP-HPLC) and Sodium Dodecyl Sulphate Polyacrilamide Gel

Electrophoresis (SDS PAGE). The results obtained through the purification of different LAB bacteriocins

(enterocin and salivaricin) carried out in our laboratory are included to discuss the different purification

strategies applied.

Keywords: bacteriocin, purification, Lactic acid bacteria, Lactobacillus, Enterococcus


(PDF) Different strategies for purification of antimicrobial peptides from Lactic Acid Bacteria (LAB). Available from: https://www.researchgate.net/publication/237411008_Different_strategies_for_purification_of_antimicrobial_peptides_from_Lactic_Acid_Bacteria_LAB [accessed Sep 11 2018].