Differential diagnosis of a pansystolic murmur.
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Atrial fibrillation, pulmonary edema, and opening snap and a diastolic rumble are prototypic findings in mitral stenosis. Holosystolic murmur. Primary MR etiology = intrinsic disease of the mitral valves and/or chordae tendineae (e.g mitral valve prolapse, myxomatous disease, endocarditis).
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Polymerase chain reaction (PCR) is a technique used to amplify a single copy or a few copies of a piece of DNA to produce multiple copies of the same sequence. PCR can be used as a diagnostic tool, where a sample of DNA is analyzed for the presence of genetic disease due to mutations.
Thus, the correct answer is 'PCR'.
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