Question

Differentiate between single immunodiffusion and double immunodiffusion

Answer 1

. Double Immune Diffusion :      

 Both Ab and Ag diffuse radially from the wells

·         As equivalence is reached a visible ring of precipitation is formed


2.  Single or Radial :

 

·         As Ag diffuses into agar, precipitant ring will form at optimal Ab-Ag concentrations

·         Comparing the area of the ring with that of a standard sample of known  Ag concentration, the test sample's Ag concentration can therefore be determined.

Answer 2

Single immunodiffusion and double immunodiffusion both are radial immunodiffusion methods.

Explanation:

Single immunodiffusion and double immunodiffusion are the methods which are used in immunology to test the antigen concentration in a sample.

Difference between single immunodiffusion and double immunodiffusion:

1. In double immunodiffusion, both antigen and antiserum together diffuses in the gel while in Single immunodiffusion only antibody is involved in the gel and only antigen diffuses in the gel.
2. In double diffusion precipitation, antigen and antibody overlaps and forms lines in the gel while in single immunodifusion, rings are formed as a result of precipitation.

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