differentiate between the premetamorphisis and prometamorphosis stages during anuran metamorphosis
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For analysis of developmental changes in whole-body corticosterone content, R. pipiens tadpoles were held in three 10 liter plastic tanks, with approximately 40 tadpoles per tank, while X. laevis tadpoles were maintained in a 170 liter holding tank as described above. Tadpoles in specific developmental stages were collected from the holding tanks over a 2-mo time period. They were anesthetized in 0.01% benzocaine immediately, weighed, placed into extraction tubes, and frozen at −20°C. Following organic extraction of the hormone (see below), whole body corticosterone content was determined by radioimmunoassay (RIA). Tadpoles were extracted either individually (for prometamorphic, climax and postclimax stages) or in pools for premetamorphic animals (R. pipiens: 4–5 tadpoles/pool; X. laevis: 3 tadpoles/pool). Sample sizes were 8 per stage for X. laevis and ranged from 3 to ,❤️❤️❤️❤️