Discuss in detail about demonstration of alkaline phosphatase
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Alkaline phosphatase (EC 3.1.3.1) or basic phosphatase is a homodimeric .... its λmax at 410 allows spectrophotometry to determine important information about enzymatic activity.
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Alkaline phosphatase or basic phosphatase is a homodimeric protein enzyme of 86 kilodaltons. Each monomer contains five cysteine residues, two zinc atoms, and one magnesium atom crucial to its catalytic function, and it is optimally active at alkaline pH environments. An alkaline phosphatase level test (ALP test) measures the amount of alkaline phosphatase enzyme in your bloodstream. The test requires a simple blood draw and is often a routine part of other blood tests. Abnormal levels of ALP in your blood most often indicate a problem with your liver, gallbladder, or bones.
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