| Discuss the importance of dose of mutagen in mutation breeding.
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USE OF MUTAGENS
- Ethyl methanesulfonate (EMS) is the most popular because of its effectiveness and ease of handling, especially its detoxification through hydrolysis for disposal
- Nitroso compounds are the other alkylating agents widely used, but they are light-sensitive and more precautions need to be taken because of their higher volatility
- EMS has become a commonly used mutagen for developing large numbers of mutants for screening such as in developing TILLING populations. Although many chemicals are mutagens, only few have been used in practical breeding as the doses need to be optimised and also because the effectiveness is not high in plants for many.
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