Discuss the significance of heavy isotope of nitrogen in the Messelson and Stahl's experiment.
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The heavy isotope of nitrogen in the band resulted in the formation of DNA had an intermediate density between N14 and N15. From this they concluded that the hybrid DNA had a strand of N14 and a strand of N15 thus proving semi conservative mode of replication
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Meselson and Stahl tested the hypothesis of DNA replication. ... 15N is a heavy isotope of nitrogen so the DNA synthesized is of heavy density. They then shifted the bacteria to a 14N medium, DNA was isolated at different times corresponding to replication cycles 0, 1, and 2....
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