Question

Distinguish between pBR322 & pUC19.

Answer 1

Here are some differences:

pUC:

- 2686 bp

- Amp-resistance gene

- High copy number plasmid

- lacZ-gene: recombinant colonies appear white, whether non-recombinants appear blue

pBR322:

- 4361 bp

- Amp- and tet-resistance genes

- Low copy number plasmid (rop gene)

- older than pUC

Hope it will helps you