Question

Dna fragmentation analysis cells will be seeded in 60 mm petri dishes at a seeding density of 4 105 cells/plate and treated with polyphenon-b (60 lg/ml) for 24 h. Cells will be harvested and washed with pbs. The oligonucleosomal dna fragments will be analyzed. Dna in the gels will be visualized under ultraviolet light after staining with ethidium bromide.

Answer 1

Dna fragmentation analysis cells will be seeded in 60 mm petri dishes at a seeding density of 4 105 cells/plate and treated with polyphenon-b (60 lg/ml) for 24 h. Cells will be harvested and washed with pbs. The oligonucleosomal dna fragments will be analyzed. Dna in the gels will be visualized under ultraviolet light after staining with ethidium bromide.