DNA precipitation out of a mixture ofbiomolecules can be achieved by treatment with
(1) Isopropanol
(2) Chilled ethanol
(3) Methanol at room temperature
(4) Chilled chloroform
Answers
Answered by
0
The DNA which has been precipitated out of bio-molecules can be seen through the addition of (2) Chilled ethanol to the mixture.
Explanation:
- The components such as DNA, RNA, and protein are considered as major bio-molecules.
- These molecules react with different compounds to show their existence in the reactant mixture.
- To get DNA precipitation, the reactant mixture must be treated with chilled ethanol.
- It is because as the dielectric constant of ethanol is lower than water, it facilitates the ionic bond formation between the salt as well as the phosphate backbone of the DNA.
- Due to this phenomenon, the DNA gets precipitated in the presence of chilled ethanol.
Learn more about such concept
Why chilled ethanol is needed for DNA precipitation?
https://brainly.in/question/5056642
Role of potassium acetate in plasmid DNA isolation
https://brainly.in/question/7137145
Similar questions