Does amount of solid sample affect uv uv visible spectroscopy
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UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.
your ans:because condensed-phase materials with high molecular density do not transmit much light in the shorter wavelength region of the UV, reflection spectroscopy has been used to ob- serve spectra of solid samples in the wavelength region shorter than 190 nm.
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