Does differential extraction involves action of heat
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The DNA extract is passed over a small column containing miniscule beads composed of a ... Using another chemical mix and mild heat, the cells are broken open, releasing the DNA.The process of liquid-liquid extraction involves the distribution of a compound between two solvents that are insoluble in each other. By taking advantage of the differing solubilities of a solute in a pair of solvents, compounds can be selectively transported from one liquid phase to the other.The first part of the differential lysis is carried out under mild conditions to gain the female DNA. Lysate of the epithelia cells is retained within the column even during heat incubation and moderate shaking. Upon centrifugation, the solution passes the column into the collection tube.
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