Biology, asked by nidhi5558, 1 year ago

Draw and explain the blood cell counter dark field method

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Answered by shivashashawat
0

https://www.google.com/url?sa=i&source=images&cd=&ved=2ahUKEwiz7MfA_7XfAhWGvI8KHQGKAZoQjRx6BAgBEAU&url=http%3A%2F%2Fblog.microscopeworld.com%2F2011%2F07%2F&psig=AOvVaw2gps11UNvvWqGM4Y9UpMuI&ust=1545656016134818rightfield microscopy uses light from the lamp source under the microscope stage to illuminate the specimen. This light is gathered in the condenser, then shaped into a cone where the apex is focused on the plane of the specimen. In order to view a specimen under a brightfield microscope, the light rays that pass through it must be changed enough in order to interfere with each other (or contrast) and therefore, build an image. At times, a specimen will have a refractive index very similar to the surrounding medium between the microscope stage and the objective lens. When this happens, the image can not be seen. In order to visualize these biological materials well, they must have a contrast caused by the proper refractive indices, or be artificially stained. Since staining can kill specimens, there are times when darkfield microscopy is used instead.

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