enlist different types of restrictions enzymes commonly used in rDna technology? write on their role.
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Type II restriction enzymes are heavily used in recombinant DNA techniques. Type II enzymes consist of single, separate proteins for restriction and modification. One enzyme recognizes and cuts DNA, the other enzyme recognizes and methylates the DNA.
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The enzymes used in the DNA recombinant DNA technology are:
Nucleases.
Ligases.
Polymerases.
Reverse transcriptase (for cDNA)
Alkaline phosphatase.
Polynucleotide kinase.
Terminal deoxynucleotidyl transferase.
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