Question

Enzyme required for removing rna primer during dna replication

Answer 1

On the lagging strand template, aprimase "reads" the template DNA and initiates synthesis of a short complementary RNA primer. A DNA polymerase extends the primed segments, forming Okazaki fragments. The RNA primers are then removed and replaced with DNA, and the fragments of DNA are joined together by DNA ligase

Answer 2

RNA primers can be removed by DNA polymerase with proofreading activity and RNAseH. Both work in different conditions. For DNA polymerase to act, it should be extending an existing template and RNA primers should be in their way. This happens during lagging strand synthesis of DNA replication. Their digestion can only happen in 5′ to 3′ direction. RNAseH work when RNA primer is bound to complementary DNA strand and has nicks in between RNA-DNA primer.

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