Biology, asked by anne6989, 1 year ago

Estimation of dna by diphenylamine method calculation

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Answered by laxmipriyaojha2002
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Answer:Introduction: The principle underlying estimation of DNA using diphenylamine is the reaction of diphenylamine with deoxyribose sugar producing blue-coloured complex. ... The ω-hydroxylevulinylaldehyde, under acidic conditions, reacts with diphenylamine to produce a blue-coloured complex that absorbs at 595 nm.

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