Question

Evaluation of drug-nucleic acid interactions by thermal melting curves

Answer 1

If the temperature of a solution containing a nucleic acid is raised sufficiencly strand seperation or melting occurs. The temperature that marks the midpoint of the melting process is called melting temperature.


At the T m, half of the nucleic acid exists in the helical state and other half exists in the single stranded state and the two species are in equilibrium.

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