Examination of Squamous Epithelium from Human Skin
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Answer:
Epithelial tissue serves two main functions in the body.
It provides linings for external and internal surfaces that face harsh environments. The outer layer of the skin is epithelial tissue, as are the innermost layers of the digestive tract, the respiratory tract, and blood vessels.
It forms glands that secrete materials onto epithelial surfaces or into the blood. Sweat glands, salivary glands, mammary glands, adrenal glands, and pituitary glands are examples of glands made of epithelial tissue.
Epithelial tissue is often classified according to numbers of layers of cells present, and by the shape of the cells. See Figure 5-1.
A simple epithelium is only one layer of cells thick. A stratified epithelium is more than one layer of cells thick. A pseudostratified epithelium is really a specialized form of a simple epithelium in which there appears at first glance to be more than one layer of epithelial cells, but a closer inspection reveals that each cell in the layer actually extends to the basolateral surface of the epithelium.
There are three basic shapes used to classify epithelial cells. A squamous epithelial cell looks flat under a microscope. A cuboidal epithelial cell looks close to a square. A columnar epithelial cell looks like a column or a tall rectangle. A few epithelial layers are constructed from cells that are said to have a transitional shape. Transitional epithelial cells are epithelial cells specialized to change shape if they are stretched laterally. They can transition from columnar- and cuboidal-looking shapes in their unstretched state to more squamous-looking shapes in their stretched state.
When classifying a stratified epithelial sheet, the sheet is named for the shape of the cells in its most superficial layers. So a stratified squamous epithelium only necessarily has squamous-shaped cells in its highest layers and might have a different-shaped cell in its lower layers.
Under a microscope, epithelial cells are readily distinguished by the following features:
The cells will usually be one of the three basic cell shapes – squamous, cuboidal, or columnar.
The cells will be closely attached to one another, in either a single layer or in multiple layers, and usually will not have room for extracellular material between the attached cells.
The epithelial layer on one side will face an empty space (or, in some organs, it will face a secreted substance like mucus) and on the other side will usually be attached to connective tissue proper.
Usually, a slide will have a section of tissue cut out of a larger organ. Slides with epithelial tissues usually have some of the underlying tissue found beneath the epithelial tissue with them.