Experiment : To show that chlorophyll is necessary for photosynthese.
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Observation : The blue colour appears only in those parts of leaf which were green in colour. The blue colour will not appear in variegated parts. This shows that starch formation takes place only in green (chlorophyll containing) parts of the leaf. Thus, the chlorophyll is essential for photosynthesis.
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In this experimental leaf, the part of the variegated leaf which contain green patches will give a positive test for starch i.e. iodine when added to this part of the leaf will turn into blue-black color. ... Thus this experiment proves chlorophyll is necessary for leaves to prepare starch through photosynthesis
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