Question

Explain a visual method of screening the transformed host cells.

Answer 1

Positive selection vectors conditionally express a lethal gene, such as a restriction enzyme that digests the genomic DNA of the bacterial host. The expression of the lethal gene is disrupted by ligation of a DNA insert into the cloning site. As a result, onlycells with recombinant plasmids are able to grow.

Answer 2

Positive selection vectors conditionally express a lethal gene, such as a restriction enzyme that digests the genomic DNA of the bacterial host. The expression of the lethal gene is disrupted by ligation of a DNA insert into the cloning site. As a result, onlycells with recombinant plasmids are able to grow.


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