explain column chromatography with different stages of seperation
Answers
Explanation:
Here are the steps involved in Column Chromatography:
1) Preparation / Packing of an adsorbent column. ...
2) Solvent system / Mobile phase. ...
3) Application of samples. ...
4) Sample elution. ...
5) Collection and analysis of fraction
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Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential adsorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions. The technique is widely applicable, as many different adsorbents (normal phase, reversed phase, or otherwise) can be used with a wide range of solvents. The technique can be used on scales from micrograms up to kilograms. The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process. The latter prevents cross-contamination and stationary phase degradation due to recycling. Column chromatography can be done using gravity to move the solvent, or using compressed gas to push the solvent through the column.