Explain how recombinant DNA is inserted into the host cell ?
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Insertion of Recombinant DNA Into Host. In this step, the recombinant DNA is introduced into a recipient host cell. This process is 'Transformation'. Bacterial cells do not accept foreign DNA easily.
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"Recombinant DNA (rDNA) can be introduced into the host cell through the following methods:
a. Transformation: The host cell directly absorbs the rDNA of the recipient cell.
b. Transfection: Chemical mediation of rDNA into the host cell.
c. Electroporation: Application of high voltage electric current to the rDNA containing solvent to enter the host cell. d. Microinjection: Introduced via micro pipette into the host nucleus.
e. Biolistics: By bombarding micro particles that are rDNA coated and targeting into the host cell.
f. Gene transfer: Transfer of rDNA is done physically, biochemically, or by vectors.
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