Question

Explain in briefly Meselson and Stahl's Experiment?

Answer 1

The Meselson–Stahl experiment is an experiment by Matthew Meselson and Franklin Stahl in 1958 which supported Watson and Crick's hypothesis that DNA replication was semiconservative. ... It has been called "the most beautiful experiment in biology."

Answer 2

Measelson and Stahl Experiment

• Measelson and Stahl transferred the E.coli with heavier DNA (N15) to a culture medium containing N14 (lighter) and allowed to produce daughter cells.

• After one generation when one E.coli were multiplied in two, they isolated the DNA.

• The DNA of daughter cell contained one heavier strand and another lighter strand.

• The heavier strand was the parent strand containing N15 and the lighter strand was newly synthesized normal strand containing N14.

• This indicated the semi conservative method of DNA replication.

• Then they allowed the daughter cell of E.coli to produce second generation.

• At the end of second generation half the DNA molecule will be hybrid and other half completely new.