Explain Lac Operon...
Answers
The lac operon consist of three structural genes , a promoter , a terminator, regulator, and an operator. The three structural genes are: lacZ, lacY, and lacA. lacZ encodes β-galactosidase (LacZ), an intracellular enzyme that cleaves the disaccharide lactose into glucose and glucatose.
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The lac operon (lactose operon) is an operon essential for human transportation and lactose metabolism and several enteric bacteria.
Explanation:
Majorly glucose is widely used as a source of carbon for most of th ebterias but in the absence of glucose, lac operon allows effective digestion with the help of beta-galactosidase activity. Gene regulation of the lac operon is considered as a prokaryotic gene regulation. François Jacob and Jacques Monod gave the classic model of lac operon on the basis of the common laboratory bacterium, E. coli,
If we discuss about the structure of lac operon, it consist 3 structural genes ( lacZ, lacY, and lacA) , regulator, a promoter, a terminator, and an operator. Only lacZ and lacY are required for lactose catabolism.
Regulation of lac operon or lac genes depends on the availability of the substrate lactose to the bacterium.
Lac opern has widely used in molecular biology in different ways such as the lac gene is used as a reporter gene in various bacterial-based selection techniques and also used in Luria broth (LB) plates having X-gal, as lac genes ( majorly lacZ and lacY) are required for lactose breakdown.
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