Question

Explain the concept of ‘salting in’ and salting out’ in enzyme purification.

Answer 1

Hi, Here is your answer

The process of "salting out" is a purification method that relies on the basis of protein solubility. It relies on the principle that most proteins are less soluble in solutions of high salt concentrations because the addition of salt ions shield proteins with multi-ion charges. There are two ways of "salting out".

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