Question

explain the procedure of observation of stomata in leaf in laboratory

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Answer 1

Answer:

1. Remove a healthy leaf from the potted plant.

2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf over and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing water.

3. Put a few drops of safranin stain in a watch glass.

4. After 2-3 minutes take out the peel and place it on a clean glass slide.

5. Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle.

6. Remove the excess stain and glycerin with the help of blotting paper.

7. Observe the slide under the low-power and high-power magnifications of the compound microscope.

Hope this may help you

Answer 2

The stomata, is a structure in leaf, which acts as  an opening for breathing of the leaf . This structure shows movements as the lungs, and also it opens and closes itself with the help of the guard cells stoma for avoiding any unwanted particle to get inside while the process of breathing.

It is observed that it shuts and opens itself in a continuous motion acting like an automatic gate.

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