Question

Explain the process of performing the confirmatory test of proteins

Answer 1

Answer:

1) To a test tube, add 40 drops of liquid to be tested.

2) If testing more than one liquid, label each test tube with a marker.

3) Add 3 drops of Biuret reagent solution to each test tube. Shake gently to mix.

4) Note any color change. Proteins will turn solution pink or purple.

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Answer 2

Explanation:

Tests that are used for confirmation include: sulfosalicylic acid (SSA); heat and acetic acid; nitric acid ring test; and Roberts' Ring Test. Any one of these procedures may be used for confirmation of the presence of protein. A protein dipstick result that is greater than a trace may be an indication of proteinuria.