Question

explain the process of slide preparation of stomata

Answer 1

1. Remove a healthy leaf from the potted plant.

2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf over and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing water.

3. Put a few drops of safranin stain in a watch glass.

4. After 2-3 minutes take out the peel and place it on a clean glass slide.

5. Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle.

6. Remove the excess stain and glycerin with the help of blotting paper.

7. Observe the slide under the low-power and high-power magnifications of the compound microscope.

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Answer 2

-Take a freshly plucked leaf, and remove the peel from its lower surface by tearing it.
-Put the leaf peel in a watch glass containing water so that the peel does not dry.
-To the watch glass containing leaf peel, add 1-2 drops of safranin to stain the peel.With the help of a brush, place the leaf peel in the centre of a clean slide.
-Put a drop of glycerine on the slide over the peel.
-Now, with the help of a needle, gently place a coverslip over the peel.Remove the excess glycerine with a blotting paper.
-Observe the slide, first, under the lower magnification (i.e., 10 X) of a compound microscope and then, under the higher magnification