Explain the separation of pure culture and discus the effect of selective nutrient on bacteria?
Answers
Culture media contains the nutrients needed to sustain a microbe.
Culture media can vary in many ingredients allowing the media to select for or against microbes.
Glucose or glycerol are often used as carbon sources, and ammonium salts or nitrates as inorganic nitrogen sources in culture media.
Explanation:
The importance of having a pure culture, and not a mixed culture, when performing biochemical testing is that a pure culture may react much differently in isolation than when it is combined with other species. Bacteria replicates at infinitesimally long rates and one species may enforce or weaken the others.
A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. ... Microbial cultures are used to determine the type of organism, its abundance in the sample being tested, or both.