explain the use of radioisotopes with reference to autoradiography and antibodies for localizing specific molecules in the cells
Answers
A variety of photographic emulsions are available, varying in sensitivity and resolution according to the size and concentration of their silver halide grains, with the least sensitive films generally offering the highest degree of resolution. Emulsions can also be obtained that are particularly sensitive to specific types of radiation.
Because radiation is emitted in all directions from the radioactive source, the greater the distance between the film and the source, the more diffuse is the resulting image. It is therefore important to use very thin samples and to place them in very close contact with the film. The highest degree of resolution is obtained with radioisotopes that emit rays of short path length and that have a high specific ionization.
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