Question

Explain the various steps involved in the production of artificial insulin?

Answer 1

Answer:

Steps involved in production of artificial insulin:

1. The plasmid of E.coli is removed and isolated using specific techniques.
2. The isolated plasmid is opened by specified enzyme.
3. In the open site of the bacterial plasmid the segment of DNA which copies for human insulin is inserted.
4. After insertion, the plasmid is covered by using another special enzyme to make it a recombinant plasmid.
5. This recombinant plasmid is again introduced into E.coli host cells.
6. Now E. coli cells become capable of synthesising human insulin. These bacteria are then cultivated in fermenters to produce a large amount of insulin.
7. Insulin is then extracted from bacterial culture and purified.

Answer 2

Human Insulin production steps are:-

1. Isolation of Donour or DNA segment
2. Formation of Recombinant DNA
3. Production of Multiple copies of rDNA.
4. Introduction of rDNA in recipient organisms.
5. Screening of Transformed cell.